Ryan Hoffman (Salama/Haussler lab)
Ryan will use stem cell-derived organoids to gain insights on the patterning role of the choroid plexus (ChP) in human cerebral cortex development. He has established a reliable protocol for the generation of ChP organoids from human induced pluripotent stem cells (hiPSCs) that form an expanding fluid filled cavity interior to developing cerebral tissue. He will perform transcriptomic and secreted proteomic analysis to determine the molecular program and the secretion profile of the human ChP across the span of embryonic development. He will then study individual molecular pathways by using known regulatory factors and/or chemical inhibitors.
The Haussler-Salama lab has been devising innovative experimental approaches using pluripotent stem cells, genome-wide measurements of gene expression and gene regulatory activity, and CRISPR/Cas9 gene editing approaches to understand how recent genome evolution contributes to human development and disease.