The IBSC Stem Cell Culture Facility is located in the Biomedical Sciences building, and is an advanced research laboratory for the culture and analysis of stem cells. This facility provides space for embryonic stem cell growth and manipulation. Facility staff offer expertise in experimental design, protocol development, and data analysis.
Facilities include multiple BSL2 culture rooms, with dedicated spaces for viral transductions and other procedures warranting a higher level of containment. The IBSC Biomedical Science facility also houses equipment required in recording images of living cells, and for applying functional genomics techniques to the study of stem cell biology.
No -80°C or liquid Nitrogen storage is offered at this time.
Fee Schedule (2022)
Please not that all fees are charged per week:
| Cell Culture | UCSC User | Non-UCSC User |
|---|---|---|
| New User Training | – | $308.00 |
| One (1) shelf | $49.50 | $125.00 |
Fee Schedule (2023)
Please not that all fees are charged per week:
| Cell Culture | UCSC User | Non-UCSC User |
|---|---|---|
| New User Training | – | $338.80 |
| One (1) shelf | $54.45 | $137.50 |
Access
The Sinsheimer TC facility is open to any UCSC researcher. The Biomedical Sciences TC facility gives priority to IBSC investigators culturing stem cells. Flow Cytometry facilities are open to any UCSC or outside research projects.
Biohazard Procedures (BSL2)
All users must have an approved BUA on file and must fill out the UCSC IBSC and SSCF safety questionnaire (PDF) before doing any project in the facility.
Training
All new cytometry and tissue culture users are expected to have taken the “Biological Agents in Shared Facilities Awareness Training” class, which is available through our campus Learning Center (login and “Find a Course” to search).
Please contact the IBSC facility manager to discuss your project and arrange facility/instrument training.
Scheduling
Cell Culture Facilities: Access to hoods in the centers is on a first-come use policy. If you will require a hood for an extended period of time on a given day, please make arrangements with the IBSC facility manager.
Cytometry Instruments: Trained users, please use the online scheduling system to reserve equipment.
Acknowledgements in publications
In all publications of research conducted in the Sinsheimer cell culture lab, please acknowledge the CIRM Shared Stem Cell Facility grant to UCSC (CL1-00506) and the facility number (RRID:SCR_021353).
In all publications of research conducted on the Biomed 4th floor (including the cell culture and virus room), in the vivarium, or using the LSR II cell analyzer/FACS Aria cell sorter, please acknowledge the CIRM Major Facility Award to UCSC (FA1-00617) and the appropriate facility number for the cell culture facility (RRID:SCR_021353) and/or the cytometry facility (RRID:SCR_021149).
Reporting Problems, Suggestions, Questions
To report problems or make suggestions concerning the Sinsheimer and Biomedmedical stem cell facilities, please email the IBSC Facility Manager. Please specify the issue in your email.
Funding/Grant Acknowledgement
This core facility is supported by the California Institute for Regenerative Medicine (CIRM) and the California Institute for Quantitative Biosciences (QB3). All publications, posters, presentations, etc. that utilize the Flow Cytometry Facility must acknowledge RRID SCR_021353.
Contact
Bari Holm Nazario, IBSC/SSCF Facility Manager
Email: bnazario@ucsc.edu
Phone: (831) 459-3980
Office: 450 Biomedical Sciences